abstract：:Sec13p has been thought to be an essential component of the COPII coat, required for exit of proteins from the yeast endoplasmic reticulum (ER). We show herein that normal function of Sec13p was not required for ER exit of the Hsp150 glycoprotein. Hsp150 was secreted to the medium under restrictive conditions in a sec...

journal_title：Molecular biology of the cell

authors： Fatal N,Suntio T,Makarow M

更新日期：2002-12-01 00:00:00

abstract：:The 260-kDa heterodimeric Gal/GalNAc-specific Lectin (Gal-lectin) of Entamoeba histolytica dissociates under reducing conditions into a heavy (hgl, 170 kDa) and a light subunit (lgl, 35 kDa). We have previously shown that inhibition of expression of the 35-kDa subunit by antisense RNA causes a decrease in virulence. T...

journal_title：Molecular biology of the cell

authors： Katz U,Ankri S,Stolarsky T,Nuchamowitz Y,Mirelman D

更新日期：2002-12-01 00:00:00

abstract：:Cyclin-dependent kinase (Cdk)2/cyclin E is imported into nuclei assembled in Xenopus egg extracts by a pathway that requires importin-alpha and -beta. Here, we identify a basic nuclear localization sequence (NLS) in the N-terminus of Xenopus cyclin E. Mutation of the NLS eliminated nuclear accumulation of both cyclin ...

journal_title：Molecular biology of the cell

authors： Moore JD,Kornbluth S,Hunt T

更新日期：2002-12-01 00:00:00

abstract：:Centrioles and basal bodies are cylinders composed of nine triplet microtubule blades that play essential roles in the centrosome and in flagellar assembly. Chlamydomonas cells with the bld2-1 mutation fail to assemble doublet and triplet microtubules and have defects in cleavage furrow placement and meiosis. Using po...

journal_title：Molecular biology of the cell

authors： Dutcher SK,Morrissette NS,Preble AM,Rackley C,Stanga J

更新日期：2002-11-01 00:00:00

abstract：:Phospholipase D (PLD) has been suggested to mediate epidermal growth factor (EGF) signaling. However, the molecular mechanism of EGF-induced PLD activation has not yet been elucidated. We investigated the importance of the phosphorylation and compartmentalization of PLD1 in EGF signaling. EGF treatment of COS-7 cells ...

journal_title：Molecular biology of the cell

authors： Han JM,Kim Y,Lee JS,Lee CS,Lee BD,Ohba M,Kuroki T,Suh PG,Ryu SH

更新日期：2002-11-01 00:00:00

abstract：:We used immunofluorescence to study the distribution and targeting of RNA polymerase (pol) III subunits and pol III transcription factors in the Xenopus laevis oocyte nucleus. Antibodies against several of these proteins stained Cajal bodies and approximately 90 specific sites on the lampbrush chromosomes. Some of the...

journal_title：Molecular biology of the cell

authors： Murphy C,Wang Z,Roeder RG,Gall JG

更新日期：2002-10-01 00:00:00

abstract：:A provisional matrix consisting of fibrin and fibronectin (FN) is deposited at sites of tissue damage and repair. This matrix serves as a scaffold for fibroblast migration into the wound where these cells deposit new matrix to replace lost or damaged tissue and eventually contract the matrix to bring the margins of th...

journal_title：Molecular biology of the cell

authors： Midwood KS,Schwarzbauer JE

更新日期：2002-10-01 00:00:00

abstract：:Phospholipase Ds (PLDs) are regulated enzymes that generate phosphatidic acid (PA), a putative second messenger implicated in the regulation of vesicular trafficking and cytoskeletal reorganization. Mast cells, when stimulated with antigen, show a dramatic alteration in their cytoskeleton and also release their secret...

journal_title：Molecular biology of the cell

authors： O'Luanaigh N,Pardo R,Fensome A,Allen-Baume V,Jones D,Holt MR,Cockcroft S

更新日期：2002-10-01 00:00:00

abstract：:In Schizosaccharomyces pombe, the Sty1 mitogen-activated protein kinase and the Atf1 transcription factor control transcriptional induction in response to elevated salt concentrations. Herein, we demonstrate that two repressors, Tup11 and Tup12, and the Prr1 transcription factor also function in the response to salt s...

journal_title：Molecular biology of the cell

authors： Greenall A,Hadcroft AP,Malakasi P,Jones N,Morgan BA,Hoffman CS,Whitehall SK

更新日期：2002-09-01 00:00:00

abstract：:Previously, we showed that spliceosomal U6 small nuclear RNA (snRNA) transiently passes through the nucleolus. Herein, we report that all individual snRNAs of the [U4/U6.U5] tri-snRNP localize to nucleoli, demonstrated by fluorescence microscopy of nucleolar preparations after injection of fluorescein-labeled snRNA in...

journal_title：Molecular biology of the cell

authors： Gerbi SA,Lange TS

更新日期：2002-09-01 00:00:00

abstract：:Phosphatidylinositol 4, 5-bisphosphate (PIP(2)) at the inner leaflet of the plasma membrane has been proposed to locally regulate the actin cytoskeleton. Indeed, recent studies that use GFP-tagged pleckstrin homology domains (GFP-PH) as fluorescent PIP(2) sensors suggest that this lipid is enriched in membrane microdo...

journal_title：Molecular biology of the cell

authors： van Rheenen J,Jalink K

更新日期：2002-09-01 00:00:00

abstract：:The export of mRNA from the nucleus to the cytoplasm involves interactions of proteins with mRNA and the nuclear pore complex. We isolated Crp79p, a novel mRNA export factor from the same synthetic lethal screen that led to the identification of spMex67p in Schizosaccharomyces pombe. Crp79p is a 710-amino-acid-long pr...

journal_title：Molecular biology of the cell

authors： Thakurta AG,Whalen WA,Yoon JH,Bharathi A,Kozak L,Whiteford C,Love DC,Hanover JA,Dhar R

更新日期：2002-08-01 00:00:00

abstract：:To investigate the molecular interactions of synaptophysin I and vesicle-associated membrane protein 2 (VAMP2)/synaptobrevin II during exocytosis, we have used time-lapse videomicroscopy to measure fluorescence resonance energy transfer in live neurons. For this purpose, fluorescent protein variants fused to synaptoph...

journal_title：Molecular biology of the cell

authors： Pennuto M,Dunlap D,Contestabile A,Benfenati F,Valtorta F

更新日期：2002-08-01 00:00:00

abstract：:CD98, an early marker of T-cell activation, is an important regulator of integrin-mediated adhesion events. Previous studies suggest that CD98 is coupled to both cellular activation and transformation and is involved in the pathogenesis of viral infection, inflammatory disease, and cancer. Understanding of the molecul...

journal_title：Molecular biology of the cell

authors： Rintoul RC,Buttery RC,Mackinnon AC,Wong WS,Mosher D,Haslett C,Sethi T

更新日期：2002-08-01 00:00:00

abstract：:EB1 proteins are ubiquitous microtubule-associated proteins involved in microtubule search and capture, regulation of microtubule dynamics, cell polarity, and chromosome stability. We have cloned a complete cDNA of Dictyostelium EB1 (DdEB1), the largest known EB1 homolog (57 kDa). Immunofluorescence analysis and expre...

journal_title：Molecular biology of the cell

authors： Rehberg M,Gräf R

更新日期：2002-07-01 00:00:00

abstract：:Insulin regulates glucose uptake into fat and muscle by modulating the distribution of the GLUT4 glucose transporter between the surface and interior of cells. The GLUT4 trafficking pathway overlaps with the general endocytic recycling pathway, but the degree and functional significance of the overlap are not known. I...

journal_title：Molecular biology of the cell

authors： Zeigerer A,Lampson MA,Karylowski O,Sabatini DD,Adesnik M,Ren M,McGraw TE

更新日期：2002-07-01 00:00:00

abstract：:Protein quality control is an essential function of the endoplasmic reticulum. Misfolded proteins unable to acquire their native conformation are retained in the endoplasmic reticulum, retro-translocated back into the cytosol, and degraded via the ubiquitin-proteasome system. We show that efficient degradation of solu...

journal_title：Molecular biology of the cell

authors： Taxis C,Vogel F,Wolf DH

更新日期：2002-06-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is a leading cause of death worldwide. Using cDNA microarrays to characterize patterns of gene expression in HCC, we found consistent differences between the expression patterns in HCC compared with those seen in nontumor liver tissues. The expression patterns in HCC were also readily di...

journal_title：Molecular biology of the cell

authors： Chen X,Cheung ST,So S,Fan ST,Barry C,Higgins J,Lai KM,Ji J,Dudoit S,Ng IO,Van De Rijn M,Botstein D,Brown PO

更新日期：2002-06-01 00:00:00

abstract：:We previously reported the identification of a novel nuclear compartment detectable in heat-shocked HeLa cells that we termed stress-induced Src-activated during mitosis nuclear body (SNB). This structure is the recruitment center for heat shock factor 1 and for a number of RNA processing factors, among a subset of Se...

journal_title：Molecular biology of the cell

authors： Denegri M,Moralli D,Rocchi M,Biggiogera M,Raimondi E,Cobianchi F,De Carli L,Riva S,Biamonti G

更新日期：2002-06-01 00:00:00

abstract：:Activation of the epidermal growth factor receptor (EGFR) triggers multiple signaling pathways and rapid endocytosis of the epidermal growth factor (EGF)-receptor complexes. To directly visualize the compartmentalization of molecules involved in the major signaling cascade, activation of Ras GTPase, we constructed fus...

journal_title：Molecular biology of the cell

authors： Jiang X,Sorkin A

更新日期：2002-05-01 00:00:00

abstract：:Members of the ADP-ribosylation factor (Arf) family of small GTPases are implicated in vesicle traffic in the secretory pathway, although their precise function remains unclear. We generated a series of 23 clustered charge-to-alanine mutations in the Arf1 protein of Saccharomyces cerevisiae to determine the portions o...

journal_title：Molecular biology of the cell

authors： Click ES,Stearns T,Botstein D

更新日期：2002-05-01 00:00:00

abstract：:HIF-1alpha is the regulated subunit of the HIF-1 transcription factor, which induces transcription of a number of genes involved in the cellular response to hypoxia. The HIF-1alpha protein is rapidly degraded in cells supplied with adequate oxygen but is stabilized in hypoxic cells. Using polysome profile analysis, we...

journal_title：Molecular biology of the cell

authors： Lang KJ,Kappel A,Goodall GJ

更新日期：2002-05-01 00:00:00

abstract：:Previously published reports support the concept that, besides promoting homotypic intercellular adhesion, cadherins may transfer intracellular signals. However, the signaling pathways triggered by cadherin clustering and their biological significance are still poorly understood. We report herein that transfection of ...

journal_title：Molecular biology of the cell

authors： Lampugnani MG,Zanetti A,Breviario F,Balconi G,Orsenigo F,Corada M,Spagnuolo R,Betson M,Braga V,Dejana E

更新日期：2002-04-01 00:00:00

abstract：:Cdc6 performs an essential role in the initiation of eukaryotic DNA replication by recruiting the minichromosome maintenance (MCM) complex onto DNA. Using immunodepletion/add-back experiments in Xenopus egg extracts, we have determined that both Walker A (ATP binding) and Walker B (ATP hydrolysis) motifs of Xenopus Cd...

journal_title：Molecular biology of the cell

authors： Frolova NS,Schek N,Tikhmyanova N,Coleman TR

更新日期：2002-04-01 00:00:00

abstract：:The yeast SIR2 gene and many of its homologs have been identified as NAD(+)-dependent histone deacetylases. To get a broader view of the relationship between the histone deacetylase activity of Sir2p and its in vivo functions we have mutated eight highly conserved residues in the core domain of SIR2. These mutations h...

journal_title：Molecular biology of the cell

authors： Armstrong CM,Kaeberlein M,Imai SI,Guarente L

更新日期：2002-04-01 00:00:00

abstract：:Here we demonstrate that multiple tetraspanin (transmembrane 4 superfamily) proteins are palmitoylated, in either the Golgi or a post-Golgi compartment. Using CD151 as a model tetraspanin, we identified and mutated intracellular N-terminal and C-terminal cysteine palmitoylation sites. Simultaneous mutations of C11, C1...

journal_title：Molecular biology of the cell

authors： Yang X,Claas C,Kraeft SK,Chen LB,Wang Z,Kreidberg JA,Hemler ME

更新日期：2002-03-01 00:00:00

abstract：:Mutations in the Caenorhabditis elegans unc-84 gene cause defects in nuclear migration and anchoring. We show that endogenous UNC-84 protein colocalizes with Ce-lamin at the nuclear envelope and that the envelope localization of UNC-84 requires Ce-lamin. We also show that during mitosis, UNC-84 remains at the nuclear ...

journal_title：Molecular biology of the cell

authors： Lee KK,Starr D,Cohen M,Liu J,Han M,Wilson KL,Gruenbaum Y

更新日期：2002-03-01 00:00:00

abstract：:Cyclins A and E and their partner cyclin-dependent kinases (Cdks) are key regulators of DNA synthesis and of mitosis. Immunofluorescence studies have shown that both cyclins are nuclear and that a proportion of cyclin A is localized to sites of DNA replication. However, recently, both cyclin A and cyclin E have been i...

journal_title：Molecular biology of the cell

authors： Jackman M,Kubota Y,den Elzen N,Hagting A,Pines J

更新日期：2002-03-01 00:00:00

abstract：:The yeast "two-component" osmotic stress phosphorelay consists of the histidine kinase, Sln1p, the phosphorelay intermediate, Ypd1p and two response regulators, Ssk1p and Skn7p, whose activities are regulated by phosphorylation of a conserved aspartyl residue in the receiver domain. Dephospho-Ssk1p leads to activation...

journal_title：Molecular biology of the cell

authors： Li S,Dean S,Li Z,Horecka J,Deschenes RJ,Fassler JS

更新日期：2002-02-01 00:00:00

abstract：:The zona pellucida (ZP) is a highly organized extracellular coat that surrounds all mammalian eggs. The mouse egg ZP is composed of three glycoproteins, called mZP1-3, that are synthesized, secreted, and assembled into a ZP exclusively by growing oocytes. Here, we microinjected epitope-tagged (Myc and Flag) cDNAs for ...

journal_title：Molecular biology of the cell

authors： Qi H,Williams Z,Wassarman PM

更新日期：2002-02-01 00:00:00

abstract：:Chediak-Higashi syndrome is a genetic disorder caused by mutations in a gene encoding a protein named LYST in humans ("lysosomal trafficking regulator") or Beige in mice. A prominent feature of this disease is the accumulation of enlarged lysosome-related granules in a variety of cells. The genome of Dictyostelium dis...

journal_title：Molecular biology of the cell

authors： Harris E,Wang N,Wu Wl WL,Weatherford A,De Lozanne A,Cardelli J

更新日期：2002-02-01 00:00:00

abstract：:We previously characterized major components of mitotic chromosomes assembled in Xenopus laevis egg extracts and collectively referred to them as Xenopus chromosome-associated polypeptides (XCAPs). They included five subunits of the condensin complex essential for chromosome condensation. In an effort to identify nove...

journal_title：Molecular biology of the cell

authors： MacCallum DE,Losada A,Kobayashi R,Hirano T

更新日期：2002-01-01 00:00:00

abstract：:The Drosophila tumor suppressor protein lethal (2) giant larvae [l(2)gl] is involved in the establishment of epithelial cell polarity during development. Recently, a yeast homolog of the protein has been shown to interact with components of the post-Golgi exocytic machinery and to regulate a late step in protein secre...

journal_title：Molecular biology of the cell

authors： Müsch A,Cohen D,Yeaman C,Nelson WJ,Rodriguez-Boulan E,Brennwald PJ

更新日期：2002-01-01 00:00:00

abstract：:Cadherin-mediated cell-cell adhesion is a dynamic process that is regulated during embryonic development, cell migration, and differentiation. Different cadherins are expressed in specific tissues consistent with their roles in cell type recognition. In this study, we examine the formation of N-cadherin-dependent cell...

journal_title：Molecular biology of the cell

authors： Mary S,Charrasse S,Meriane M,Comunale F,Travo P,Blangy A,Gauthier-Rouvière C

更新日期：2002-01-01 00:00:00

abstract：:DSL1 was identified through its genetic interaction with SLY1, which encodes a t-SNARE-interacting protein that functions in endoplasmic reticulum (ER)-to-Golgi traffic. Conditional dsl1 mutants exhibit a block in ER-to-Golgi traffic at the restrictive temperature. Here, we show that dsl1 mutants are defective for ret...

journal_title：Molecular biology of the cell

authors： Reilly BA,Kraynack BA,VanRheenen SM,Waters MG

更新日期：2001-12-01 00:00:00

abstract：:The kinesin superfamily of microtubule motor proteins is important in many cellular processes, including mitosis and meiosis, vesicle transport, and the establishment and maintenance of cell polarity. We have characterized two related kinesins in fission yeast, klp5+ and klp6+,, that are amino-terminal motors of the K...

journal_title：Molecular biology of the cell

authors： West RR,Malmstrom T,Troxell CL,McIntosh JR

更新日期：2001-12-01 00:00:00

abstract：:We applied a combination of laser microsurgery and quantitative polarization microscopy to study kinetochore-independent forces that act on chromosome arms during meiosis in crane fly spermatocytes. When chromosome arms located within one of the half-spindles during prometa- or metaphase were cut with the laser, the a...

journal_title：Molecular biology of the cell

authors： LaFountain JR Jr,Oldenbourg R,Cole RW,Rieder CL

更新日期：2001-12-01 00:00:00

abstract：:The Forkhead family of transcription factors participates in the induction of death-related genes. In NMuMG and 4T1 mammary epithelial cells, transforming growth factor beta (TGF beta) induced phosphorylation and cytoplasmic retention of the Forkhead factor FKHRL1, while reducing FHKRL1-dependent transcriptional activ...

journal_title：Molecular biology of the cell

authors： Shin I,Bakin AV,Rodeck U,Brunet A,Arteaga CL

更新日期：2001-11-01 00:00:00

abstract：:Melanosomes are tissue-specific organelles within which melanin is synthesized and stored. The melanocyte-specific glycoprotein Pmel17 is enriched in the lumen of premelanosomes, where it associates with characteristic striations of unknown composition upon which melanin is deposited. However, Pmel17 is synthesized as...

journal_title：Molecular biology of the cell

authors： Berson JF,Harper DC,Tenza D,Raposo G,Marks MS

更新日期：2001-11-01 00:00:00

abstract：:We have previously identified a novel family of proteins called the GGAs (Golgi-localized, gamma-ear-containing, ADP-ribosylation factor-binding proteins). These proteins consist of an NH(2)-terminal VHS domain, followed by a GAT domain, a variable domain, and a gamma-adaptin ear homology domain. Studies from our own ...

journal_title：Molecular biology of the cell

authors： Hirst J,Lindsay MR,Robinson MS

更新日期：2001-11-01 00:00:00